113 - John Isner (Wimbledon 2010) 5 sets, defeated Nicolas Mahut
103 - Nicolas Mahut (Wimbledon 2010) 5 sets, lost to John Isner
78 - Ivo Karlovic (Davis Cup 2009) 5 sets, lost to Radek Stepanek
55 - Ivo Karlovic (Roland Garros 2009) 5 sets, lost to Lleyton Hewitt
54 - Gary Muller (Wimbledon 1993, qualifying match) 3 sets, defeated Peter Lundgren
51 - Joachim Johansson (Australian Open 2005 ) 4 sets, lost to Andre Agassi
51 - Ivo Karlovic (Wimbledon 2005) 5 sets, lost to Daniele Bracciali
50 - Chris Guccione (Wimbledon 2005, qualifying match) 3 sets, defeated Olivier Patience
50 - Gregory Carraz (Andrézieux 2004 - Challenger) 3 sets, defeated Tomas Zib
50 - Roger Federer (Wimbledon 2009) 5 sets, defeated Andy Roddick
The first and the second place are from the longest match ever played. It was John Isner vs Nicolas Mahut at Wimbledon in 2010. With that many aces, it is easy to see why the score in the 5th set was 70-68, and the match took 11 hours and 5 minutes. They just kept winning on their serves.
